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Peptide Half-Life Calculator

Enter a half-life and elapsed time to see what percentage of the molecule remains. Pure pharmacokinetic arithmetic — the formula is shown.

By The Health Stacks Research Team

25% remaining

After 2 half-lives, approximately 75% of the molecule has been eliminated.

Washout reference

Approximate elimination for a half-life of 120 minutes.

Half-livesTime elapsedRemainingEliminated
12 hr50%50%
24 hr25%75%
36 hr12.5%87.5%
48 hr6.3%93.8%
510 hr3.1%96.9%

Pharmacokinetic arithmetic for research purposes — not medical advice. Actual elimination depends on individual metabolism, route of administration, and many other factors. The formula used: remaining = 0.5^(elapsed / half-life). This assumes first-order elimination.

Common peptide half-lives

Approximate half-lives from published literature. These are averages — individual results vary significantly by route, formulation, and biology.

PeptideApproximate half-lifeSource
BPC-157Several hours (oral) / stable (local)Varies by route
TB-500 (Thymosin β4)~2 weeksPublished PK estimates
Semaglutide~1 weekNovo Nordisk PI
Tirzepatide~5 daysEli Lilly PI
Epitalon~12 minutesPublished PK
GHK-Cu~seconds to minutes (in serum)Copper-binding kinetics

Common questions

What is a half-life?

A half-life is the time it takes for half of a substance to be eliminated from the body (or a system). After one half-life, 50% remains. After two, 25%. After five, about 3%. The calculator uses the first-order elimination formula: remaining = 0.5^(elapsed / half-life).

How many half-lives does it take for a peptide to clear?

A common reference point is 5 half-lives, at which point roughly 97% of the molecule has been eliminated. The exact time depends on the specific peptide’s half-life. For a peptide with a 2-hour half-life, that is about 10 hours; for one with a 7-day half-life, that is about 35 days.

Does this account for individual metabolism?

No. This is the standard pharmacokinetic formula for first-order elimination, which is a mathematical model. Real-world elimination varies between individuals based on body composition, organ function, route of administration, and other factors. This is research-use arithmetic, not a clinical prediction.

What is first-order elimination?

First-order elimination means the rate of elimination is proportional to how much of the substance is present. Each half-life, the amount drops by 50% regardless of the starting amount. This is the standard model for most peptides and small molecules, though some substances follow different kinetics.

Educational content only. The Health Stacks does not provide medical advice. Peptides discussed on this site are not FDA-approved for human use unless explicitly stated. Always consult a qualified healthcare professional before use.
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